Deteksi Turnip Mosaic Virus (TuMV) pada Tanaman Brokoli di Boyolali, Indonesia: Deteksi Turnip Mosaic Virus (TuMV) pada Tanaman Brokoli di Boyolali, Indonesia

Wiwit Probowati; Pilar Rosatria Firyalunfah; Tsania Taskia Nabila

doi:10.33019/e80fdb13

Penulis

Wiwit Probowati Universitas 'Aisyiyah Yogyakarta
Pilar Rosatria Firyalunfah Universitas 'Aisyiyah Yogyakarta
Tsania Taskia Nabila Universitas 'Aisyiyah Yogyakarta

DOI:

https://doi.org/10.33019/e80fdb13

Kata Kunci:

Brokoli, Deteksi, TuMV

Abstrak

Turnip mosaic virus (TuMV) adalah salah satu virus tanaman yang menyebabkan kerugian panen pada tanaman sayuran jenis brassica, termasuk di Indonesia. Brokoli (Brassica oleracea var. Italica) yang merupakan komoditas penting sayuran di daerah dataran tinggi Jawa Tengah memiliki masalah terkait produktivitas menurun dikarenakan penyakit yang disebabkan oleh infeksi virus. Tujuan dari penelitian ini adalah untuk mendeteksi TuMV yang menginfeksi brokoli dengan menggunakan teknik reverse transcription polymerase chain reaction (RT-PCR). Survei lapangan telah dilakukan untuk mengetahui gejala penyakit di kebun brokoli di Boyolali, Jawa Tengah. Penelitian ini dilakukan melalui beberapa tahap, yaitu: pengambilan sampel daun di lapangan, isolasi RNA virus, RT-PCR, dan deteksi dengan menggunakan primer spesifik coat protein (CP). Hasil pengamatan di lapangan ditemukan tanaman brokoli yang menunjukkan beberapa gejala infeksi TuMV, seperti: gejala mosaik daun, daun melepuh, urat daun melebar, urat daun terbuka, dan bercak daun menguning. Deteksi TuMV dengan RT-PCR menunjukkan bahwa brokoli dengan gejala-gejala tersebut positif terinfeksi TuMV. Pita DNA spesifik teramplifikasi dari tanaman terinfeksi terdeteksi pada 800 bp. Penelitian ini melaporkan adanya infeksi TuMV secara alami pada tanaman brokoli dengan gejala-gejala tersebut di Boyolali, Jawa Tengah, Indonesia.

Unduhan

Data unduhan tidak tersedia.

Biografi Penulis

Wiwit Probowati, Universitas 'Aisyiyah Yogyakarta

Program Studi Bioteknologi, Fakultas Sains dan Teknologi
Pilar Rosatria Firyalunfah, Universitas 'Aisyiyah Yogyakarta

Program Studi Bioteknologi, Fakultas Sains dan Teknologi
Tsania Taskia Nabila, Universitas 'Aisyiyah Yogyakarta

Program Studi Bioteknologi, Fakultas Sains dan Teknologi

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Deteksi Turnip Mosaic Virus (TuMV) pada Tanaman Brokoli di Boyolali, Indonesia